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. 2009 Jul 13;4(7):e6221. doi: 10.1371/journal.pone.0006221

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A, A single fura-2 loaded and Ad-MtLuc-RFP-infected mouse β-cell was imaged to determine the [Ca²⁺]_I, at 5.6 mM glucose in KRBH. After establishment of a stable baseline [Ca²⁺]_i, 10 nM Ex-4 was applied for 25 sec (indicated by horizontal bars). Note that a repeatable increase of [Ca²⁺]_i was measured. B, Population study conducted at the single cell level in which the action of Ex-4 to increase [Ca²⁺]_i was evaluated in β-cells not infected (open bars) or infected with Ad-MtLuc-RFP (filled bars). For these experiments, the KRB contained 5.6 or 7.5 mM glucose, as indicated. A response to Ex-4 was defined as a >100 nM increase of [Ca²⁺]_i occurring in a single β-cell. C, Ex-4 caused a dose-dependent increase in cAMP content in mouse islet cells in KRB containing 7.5 mM glucose without or with Ad-MtLuc-RFP infection.