The regulation of apical complexes is a specific downstream effect of the
Hippo pathway. (A,B) Tsc1 mutant clones marked by the absence of GFP
expression (green in A and B) had normal levels of aPKC and Patj (red in A, B
and grayscale in A′, B′) in eye (A) and wing (B) discs. (C-F) aPKC
levels (red in the overlay channels, grayscale in C′, D′,E′
and F′) were not affected in wing discs overexpressing Myc (C), CycD and
Cdk4 (D), the bantam miRNA (E), or CycE and DIAP1 (F) in their
posterior compartments under the control of hh-Gal4. Anterior
compartments are marked with Ci labeling (green in C, D, E and F). Anterior is
to the left and dorsal is up for all discs. Arrowheads point to
anterior-posterior compartment boundaries. Scale bars: 100 μm.