Fig. 4.

Accumulation of apical complexes in warts mutant cells leads to apical domain expansion. (A-F) Third instar wing imaginal discs containing wts mutant clones marked by the absence of β-gal expression (blue in A-F and gray in A′″-D′″). Discs were stained for the apical markers Patj (green in A,B and grey in A″,B″) and Sas (green in C,D, red in E,F and grey in C″,D″), the basolateral marker Dlg (red in A-D and grey in A′-D′), and the adherens junction marker E-cad (green in E,F and grey in E′,F′). (G-K) Crb (red in G,H and green in G′,H′) and aPKC levels (green in I-L and grey in I′-L′) were elevated in wing discs overexpressing Yki (G-J) and Crb (K,L) in their posterior compartments under the control of hh-Gal4. Posterior compartments are marked by coexpression of GFP (green in G,H). Anterior compartments are marked by Ci labeling (red in I-L). (B,D,F,H,J,L) Optical sections are shown through the wing pouch of the corresponding discs above them. Arrowheads mark clone borders. Scale bars: 50 μm.