Fig. 1.

Expression and subcellular localization of HA-tagged wt PIP5K-Iβ and catalytically inactive mutant. (A) Cells expressing the wt (IβWT) or mutant (IβMut) kinases were identified on the basis of EGFP expression via an IRES sequence in the vector (left panels). 2H3, untransfected RBL-2H3 cells. The HA-tag was labeled in fixed and permeabilized cells (right panels). (B) wt and mutant PIP5K-Iβ were immunoprecipitated from three individual IβWT and two IβMut clones using rabbit polyclonal anti-HA antibody or monoclonal anti-HA for detection on blots. EGFP expression was confirmed by immunoprecipitation and detected using an anti-GFP mAb.