Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cytokine. 2009 Apr 11;46(3):351–358. doi: 10.1016/j.cyto.2009.03.007

Figure 1. IL-32 is present in human endothelial cells and Akt induces its expression.

Figure 1

HUVECs were infected with control Ad β-gal (lane 2) or AdAkt (lane 3) adenoviral vector (MOI=10), respectively, for 48 hours. Uninfected cells were used as a control (lane 1). IL-32 mRNA was analyzed by Northern blot. 18S rRNA, visualized with ethidium bromide and UV, was used as a loading control (Panel A). IL-32 was amplified using a human tissue cDNA panel by PCR, and G3PDH was used as an internal control (Panel B). Each experiment was repeated at least twice. Representative images were shown.