Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cytokine. 2009 Apr 11;46(3):351–358. doi: 10.1016/j.cyto.2009.03.007

Figure 3. IL-32 promoter analysis.

Figure 3

Various IL-32 promoter constructs were cloned into a pGL3 vector in front of a luciferase (Luc) reporter (Panel A). +1 is the original reported transcription start site. These constructs were transfected with an internal control plasmid expressing renilla luciferase into NIH3T3 cells for 24 hours, followed by measuring luciferase activities. Transfection efficiency was normalized using renilla luciferase unit. Promoter activity as calculated as fold changes over empty vector pGL3 transfected cells (Panel B). *p<0.05, **p<0.01. Each experiment was repeated at least three times.