Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cytokine. 2009 Apr 11;46(3):351–358. doi: 10.1016/j.cyto.2009.03.007

Figure 4. Determination of IL-32 transcription start site in endothelial cells.

Figure 4

HUVECs were treated with IL-1β at 100 pg/ml for 4 hours to induce IL-32 transcription. Total RNA was isolated and subjected to the RACE reaction. The PCR products were cloned and sequenced. +1 is the original reported transcription start site. Arrows indicate the identified transcription start site, which is 328bp downstream from the original reported transcription start site. The underlined sequence represents a TATA binding site. Bold letters represent the translational initiation site.