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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cytokine. 2009 Apr 11;46(3):351–358. doi: 10.1016/j.cyto.2009.03.007

Figure 5. Identification of a downstream sequence from the transcription start site important for promoter activity.

Figure 5

A 172bp deletion downstream from the identified transcription site was generated based on the minimum promoter construct (−0.2kb to +0.5kb) (Panel A). +1 is the original reported transcription start site. Arrows indicate the newly identified transcription start site. These constructs were transfected into NIH3T3 cells, and promoter activity was analyzed as described in Figure 3 (Panel B). The minimum promoter construct (−0.2kb to +0.5kb) was transfected into NIH3T3 cells or HUVECs and the promoter activity was compared between the two cell types (Panel C). *p<0.05, **p<0.01. Each experiment was repeated at least three times.