Figure 5. Compound 6c-induced neurotrophic effect is independent of glial proliferation.

Proliferation in mesencephalic cultures was not affected by compound 6c treatment (d) compared to non-treated cultures (CTL) (a). EGF at 20 ng/mL (b) and arabinoside-C (ARA-C) at 2 µM (c), respectively, were used as controls for activation or inhibition of cell proliferation. [³H]-thymidine was added to the cultures for 18 h from DIV7 to DIV8. Proliferation was assessed by counting [³H]-thymidine⁺ nuclei appearing from autoradiographic emulsion. Images were acquired with a phase-contrast microscope coupled to a digital camera. Values are normalized to the non-treated cultures (CTL) and represent the average of three assays performed in triplicate and expressed as mean±SEM.