Fig. 7.
Binding of GalMBP to a panel of breast cancer cell lines and purification of glycoprotein ligands. (A and B) Binding of GalMBP was assayed as described in Figure 2A. (C and D) Gel electrophoresis of GalMBP ligands from multiple tumor cell lines purified by affinity chromatography on immobilized GalMBP. (C) Gels (17.5%) were stained with Coomassie blue or blotted onto nitrocellulose and probed with monoclonal anti-Lewisx antibodies followed by the peroxidase-conjugated goat anti-mouse secondary antibody. (D) Gels (5%) were blotted on nitrocellulose and probed with the monoclonal anti-MUC1 antibody followed by the peroxidase-conjugated goat anti-mouse secondary antibody.