Widespread laminar specific effects upon the cortical mantle of Cln5 deficient mice. (A-D) Laminar thickness measurements in primary motor (M1, A), primary somatosensory (S1BF, B), primary visual (V1, C) and lateral entorhinal (LEnt, D) cortex revealed a complex series of changes in the thickness of individual laminae in 12 month old Cln5-/- mice compared with littermate controls. Consistent across all cortical regions mutant mice displayed significant thinning of the deeper laminae (IV-VI), but a trend towards increased thickness in more superficial laminae (I-III) that was less pronounced in LEnt. (mean±SEM; * p<0.05; ** p<0.01; *** p<0.001; ANOVA with post-hoc Bonferroni analysis).