Muscarinic agents and Growth factor activity. A: EGF-R ELISA of mouse scleral fibroblast cell lysate after 24 h exposure to atropine and carbachol at 0, 0.1, 1, 10, and 100 µM found a dose-dependent change of EGF receptor in the presence of muscarinic agents. Carbachol stimulated EGF receptor activation in the cultured SFs. In contrast, the EGF receptor activation was inhibited by atropine in a dose-dependent manner (p<0.01, ANOVA, n=4). B: The results of TGF-β1 ELISA of mouse scleral fibroblast cell lysate after 24 h exposure to atropine and carbachol at 0, 0.1, 1, 10, and 100 µM is illustrated on the graph. Carbachol stimulated TGF-β1 activation in the cultured SFs. In contrast, the TGF-β1 activation was inhibited by atropine in a dose-dependent manner (p<0.01, ANOVA, n=4). Data are represented as mean±SEM.