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. 2009 Jun 30;15:1277–1293.

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Gene expression of muscarinic receptor subtypes A: RT–PCR identified cDNA for M₁-M₅ in human scleral fibroblasts. Lane 1: DNA ladder; lane 2: M₁; lane 3: M₂; lane 4: M₃; lane 5: M₄; lane 6: M₅. PCR products of the expected sizes were as follows: M₁, 538 bp; M₂, 654 bp; M₃, 560 bp; M₄, 503 bp; and M₅, 752 bp. The identity of the products was confirmed by sequencing. B: RT–PCR identified cDNA for M₁-M₅ in mouse scleral fibroblasts. Lane 1: DNA ladder; lane 2: mouse brain as a positive control; lane 3: mouse sclera. PCR products of the expected sizes were as follows: M₁, 139 bp; M₂, 69 bp; M₃, 91 bp; M₄, 122 bp; and M₅, 76 bp. C: β-actin as internal control, 314 bp for all cDNAs used. Lane 1: DNA ladder; lane 2: mouse brain; lane 3: human sclera; lane 4: mouse sclera. D: Water was used as a negative control. Lane 1: DNA ladder; lane 2: M₁; lane 3: M₂; lane 4: M₃; lane 5: M₄; lane 6: M₅. Identity of the products was confirmed by sequencing (see Methods for detailed information).