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Effect of muscarinic agents on MAPK signaling proteins. Phospho-EGF-R, PKC, Pyk2, B-Raf, Ras, JNK1/2 and ERK1/2activity was detected by immunoblot in the cultured mouse SF cells after being treated with atropine, pirenzepine, and carbachol at a concentration of 50 µM and with MT-7 and MT-1 at a concentration of 0.5 µM all for 30 min. β-tubulin was used as a loading control for all the treatment. Antagonists increased their activation, and agonists reversed this.
