Table 2. Primer sequences and sizes of human PCR products.
| Gene | Primers | Size |
|---|---|---|
|
M1 |
F: 5′-CAGGCAACCTGCTGGTACTC-3′ |
538 bp |
| R: 5′-CAGGCAACCTGCTGGTACTC-3′ |
||
|
M2 |
F: 5′-CTCCTCTAACAATAGCCTGG-3′ |
654 bp |
| R: 5′-GGCTCCTTCTTGTCCTTCTT-3′ |
||
|
M3 |
F: 5′-GGACAGAGGCAGAGACAGAA-3′ |
560 bp |
| R: 5′-GAAGGACAGAGGTAGAGTGG-3′ |
||
|
M4 |
F: 5′-ATCGCTATGAGACGGTGGAA-3′ |
503 bp |
| R: 5′-GTTGGACAGGAACTGGATGA-3′ |
||
|
M5 |
F: 5′-ACCACAATGCAACCACCGTC-3′ |
752 bp |
| R: 5′-ACAGCGCAAGCAGGATCTGA-3′ |
||
|
β-actin |
F: 5′-CACTCTTCCAGCCTTCCTTC-3′ |
314 bp |
| R: 5′-CTCGTCATACTCCTGCTTGC-3′ |
The β-actin PCR primer pair was selected to span a 206 bp intron [61].