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. 2009 Apr;82(4):277–287. doi: 10.1111/j.1600-0609.2009.01212.x

Figure 3.

Figure 3

Inhibition of c-Met signaling reduced IL-6-induced proliferation in ANBL-6 cells. (A and B) ANBL-6 cells were grown in serum-free media for 3 d with combinations of different concentrations of IL-6 and the c-Met tyrosine kinase inhibitor PHA-665752 or 10 μg/mL of an antibody blocking HGF binding to c-Met or 10 μg/mL of a control antibody of the same isotype before estimation of DNA synthesis. The concentration of PHA-665752 in B was 200 nm. Error bars represent SEM of triplicate measurements. In (A) incorporation of thymidine at 50 and 100 nm PHA-665752 were statistically significant different from the control at IL-6 concentrations above 0.1 ng/mL. In (B) * denotes statistically significant difference from the control or IgG-treatment (P< 0.05). (C) ANBL-6 cells were grown in serum-free media with or without 1 ng/mL IL-6 over night. Immunoblots were probed with antibodies against c-Met and GAPDH.

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