Fig. 1.

Comparison of IMAC-enriched proteins from cell lysate of Haloferax volcanii wild-type (WT) and panA deletion (ΔpanA) strains. Proteins enriched from cell lysate by IMAC were separated by reducing 12% SDS-PAGE and stained with SYPRO Ruby as described previously (Kirkland et al., 2008). Regions of reproducible and differential protein banding between the two strains such as the 25–30 kDa region shown were digested with trypsin and analyzed by MS/MS. PCNA DNA-sliding clamp protein of 27 kDa was identified by this semi-quantitative 1-D-gel approach.