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. 2009 Jul 14;4(7):e6229. doi: 10.1371/journal.pone.0006229

Figure 1. Assay sensitivity.

Figure 1

RNA miRNA analog oligonucleotides, at concentrations ranging from 0 to 40 million copies per microliter, were spiked into 400 ul of serum after the addition of RLT buffer. RNA was then extracted from the serum using phenol/chloroform extractions and an ethanol precipitation. Samples were then labeled and hybridized on a microarray. Vertical bars indicate array signal intensities for specific miRNA probes representing the wild type sequence (Wild) and probes with two internal mutations (mut) for (A) oar|miR-431 and (B) oar|miR-127. Scales for the 4,000 and 0 copies data points (boxed in left panels) are expanded in the right panels: (C) oar|miR-431, and (D) oar|miR-127.