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. Author manuscript; available in PMC: 2009 Jul 2.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2008 Dec 18;29(2):217–224. doi: 10.1161/ATVBAHA.108.181024

Figure 6.

Figure 6

Requirement of G12/13 activity for AngII-induced ROCK activation and VSMCs migration. A, The cells were infected with adenovirus encoding p115RGS, a selective G12/13 inhibitor, or its control vector (50 moi) for 48 hours and stimulated with 100 nmol/L AngII for 2 minutes. The cell lysates were subjected to immunoblot analysis with antibodies as indicated (n=3). B and C, Confluent VSMCs infected with adenovirus encoding (B) eNOS or the control vector (20 moi) or (C) p115RGS or the control vector (50 moi) were scraped by a metal dental pick and stimulated with AngII for 24 hours in the presence of 5 mmol/L hydroxyurea to block cell proliferation completely. The nucleus was stained with Hoechst 33342 dye, and migrated VSMCs from the wound edge were counted in 4 independent view fields (100×). Results shown are representatives from 3 independent experiments (each n=4).