Table 1.
Strains used in this study
| Description | Reference or source | |
|---|---|---|
| V. cholerae strains | ||
| AC304 | El Tor N16961, SmR | (Angelichio et al., 1999) |
| AC390 | El Tor N16961, SmR lacZ::res-tet-res | Laboratory strain |
| KFV21 | N16961 rpoS::KnR SmR | Gift of Karla Satchell |
| AC1526 | N16961 ΔglgC1 | This work |
| LB5 | N16961 ΔglgC1 (pTrc99A::glgC1) | This work |
| LB6 | N16961 ΔglgC1 (pTrc99A::glgC2) | This work |
| AC1527 | N16961 ΔglgC2 | This work |
| LB7 | N16961 ΔglgC2 (pTrc99A::glgC2) | This work |
| AC1528 | N16961 ΔglgC1 ΔglgC2 | This work |
| LB8 | N16961 ΔglgC1 ΔglgC2 (pTrc99A::glgC1) | This work |
| LB9 | N16961 ΔglgC1 ΔglgC2 (pTrc99A::glgC2) | This work |
| LB12 | N16961 ΔglgX | This work |
|
| ||
| E. coli strains | ||
| DH5α | F-Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 suupE44thi-1 gyrA96 relaA1 | Laboratory strain |
| DH5αλpir | F-Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 suupE44thi-1 gyrA96 relaA1 λ::pir | Laboratory strain |
| SM10λpir | thi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pir | Laboratory strain |
| AC1516 | E. coli DH5α (pCR-Script AmpK+::ΔglgC1). ApR | This work |
| AC1517 | E. coli DH5α (pCR-Script AmpK+::ΔglgC2). ApR | This work |
| AC1518 | E. coli DH5αλpir (pCVD442lac::ΔglgC1). ApR | This work |
| AC1519 | E. coli DH5αλpir (pCVD442lac::ΔglgC2). ApR | This work |
| AC1522 | E. coli SM10λpir (pCVD442lac::ΔglgC2). ApR | This work |
| AC1523 | E. coli Sm10λpir (pCVD442lac::ΔglgC1). ApR | This work |
| AC2393 | E. coli DH5α (pTrc99a). ApR | Laboratory strain |
| LB1 | E. coli DH5α (pTrc99a::glgC1). ApR | This work |
| LB2 | E. coli DH5α (pTrc99a::glgC2). ApR | This work |
| LB3 | E. coli SM10λpir (pTrc99a::glgC1). ApR | This work |
| LB4 | E. coli SM10λpir (pTrc99a::glgC2). ApR | This work |
| LB10 | E. coli DH5α (pCR-Script AmpK+::ΔglgX). ApR | This work |
| LB11 | E. coli DH5αλpir (pCVD442lac::ΔglgX). ApR | This work |