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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Nat Med. 2009 Jun;15(6):649–656. doi: 10.1038/nm.1958

Figure 2.

Figure 2

CyPA deficiency reduces AngII-induced inflammatory cell accumulation and microvessel formation. (a,b) Representative CD45 staining of suprarenal aorta from Apoe−/− and Apoe−/−Ppia−/− mice infused with AngII for 4 weeks. (c) Number of migrating CD45+ cells in the aortic wall in Apoe−/−(n = 9) and Apoe−/−Ppia−/− (n = 7) mice. (d–g) Representative immunostaining of α-smooth muscle actin (α-SMA) and Ki67 in suprarenal aorta. (h) Number of proliferating microvessels in the aortic wall. Results are mean ± SD. ★P < 0.01 compared with Apoe−/− mice. (i) CyPA is secreted from mouse VSMC in response to AngII. Pretreatment with Rho kinase inhibitor Y27632 (30 µM) and simvastatin (30 µM) for 30 min reduced CyPA secretion. CM, conditioned media; TCL, total cell lysate.