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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Nat Med. 2009 Jun;15(6):649–656. doi: 10.1038/nm.1958

Figure 4.

Figure 4

CyPA is crucial for secretion and activation of MMPs. (a) Representative western blot of MT1-MMP expression in mouse aorta after 7 d infusion of AngII. (b) Gelatin zymography for conditioned media from whole aorta organ culture. Aortas from Apoe−/− and Apoe−/−Ppia−/− mice infused with saline or AngII were incubated in media for 20 h. (c) In situ zymography for gelatinase activity. Aortas from Apoe−/− and Apoe−/−Ppia−/− mice infused with saline or AngII for 7 days were analysed. Scale bars, 100 µm. (d) Densitometric analysis of MMP activity (DQ gelatin) changes relative to the density of MMP activity in control Apoe−/− mice (saline-infused). Results are mean ± SD. ★P < 0.01 compared with Apoe−/− mice. (e) Gelatin zymography for VSMC harvested separately from the thoracic aorta (T), suprarenal aorta (S), and infrarenal aorta (I) of Apoe−/− and Apoe−/−Ppia−/− mice. VSMC from Apoe−/− and Apoe−/−Ppia−/− mice harvested from different portions of aorta were stimulated with AngII (1 µM) for 24 h. (f) Representative in situ zymography (DQ gelatin) of Ppia+/+ VSMC and immunostaining with α-tubulin after stimulation with CyPA (100 nM) for 4 h. (g) Densitometric analysis of MMP activity changes relative to the density of MMP activity in control VSMC. ★P < 0.01 vs. control VSMC. Results are mean ± SD of 6 independent experiments.