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. 2009 Jul;150(3):1135–1146. doi: 10.1104/pp.109.138214

Figure 6.

Figure 6.

Summarized results of EST assignment to TriFLCDSs on the basis of similarity searches, and a typical example of clustered ESTs assembled together with full-length CDSs to form a contig sequence. Pairwise alignments between ESTs and possible full-length counterpart TriFLCDSs with at least 80% identity and a HSP of at least 100 bp were plotted under each of the search conditions. The BLAST-searched combinations of queried ESTs and full-length CDSs indicated by asterisks (*) in the graph were used for sequence assembly against the wheat and barley entries provided in TriFLDB. TaEST and HvEST refer to the EST datasets of wheat and barley, respectively, that were used as query data in the BLAST search. TaFLCDS and HvFLCDS refer to the TriFLCDS entries for wheat and barley, respectively, that were used as the database in the BLAST search (A). Barley and wheat ESTs assigned to the TriFLCDSs of barley and wheat, respectively, were assembled, and each of these is shown in TriFLDB. A barley entry, AK251905, is shown as an example of the assembly of barley ESTs and TriFLCDS (B). [See online article for color version of this figure.]