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. 2009 Jul;150(3):1345–1355. doi: 10.1104/pp.109.137174

Figure 3.

Figure 3.

Growth impairment and ABA-hypersensitive growth inhibition of triple hab1-1abi1-2abi2-2 (triple abi2-2) and hab1-1abi1-2pp2ca-1 (triple pp2ca-1) mutants as compared to wild type. A, qRT-PCR analysis of HAB1, PP2CA, ABI1, and ABI2 transcript levels in wild type and triple hab1-1abi1-2abi2-2 and hab1-1abi1-2pp2ca-1 mutants. Gene expression was analyzed in mRNAs extracted from 14-d-old seedlings treated with 10 μm ABA for 3 h. Data are averages ± sd from three independent experiments. B, Reduced growth in both Murashige and Skoog (MS) medium and soil of triple mutants compared to wild type. The photographs were taken after 3 weeks of plant growth in Murashige and Skoog medium or soil. The graphics show the quantification of fresh weight (n = 30) and leaf area (n = 10) in triple mutants with respect to wild type. Data are averages ± sd from two independent experiments. *, P < 0.01 (Student's t test) when comparing data from the mutant and wild type in the same assay conditions. C, ABA-hypersensitive root growth inhibition of double and triple mutants compared to wild type and single parental mutants. Representative seedlings were selected and a photograph was taken after 7 d of growth in medium lacking or supplemented with 10 μm ABA. Data are averages ± sd from three independent experiments (n = 15). *, P < 0.01 (Student's t test) when comparing data from the mutant and wild type in the same assay conditions. [See online article for color version of this figure.]