Membrane lipid composition of DGD-deficient mutants carrying the Chloroflexus GlcT gene. Lipids were isolated from leaves of plants grown in the presence or absence of phosphate (+P, black bars; –P, gray bars) and separated by TLC. After transmethylation, fatty acid methyl esters were quantified by GC. SQD and phosphatidylinositol (PI) were not separated. The bars show lipid composition in mol % and indicate means ± sd of three measurements. n.d., Nondetectable (below 1 mol %); PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; WT, wild type.