Figure 8.

Role of AUX1 and the TIR1 in mediating Trp conjugate activity. A and B, Root lengths for aux1 and tir1 were determined 5 and 7 d, respectively, after growth in medium containing the indicated amounts of IAA and Trp conjugates. Values are the mean of 17 and 19 seedlings for experiments with aux1 and tir1, respectively, with se indicated. Asterisks in B indicate means that differed significantly between the two genotypes for a given treatment (t test, P < 0.01). C, Pull down of TIR1-myc with GST-Aux/IAA7. IAA was added to the interaction mix at the concentrations indicated, and JA-Trp or IAA-Trp was added at 40 mm. The bottom panel is a loading control for Aux/IAA7 stained with Ponceau Red.