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Molecular analysis of Arabidopsis wild-type (Col-0) and 35S:FsGASA4 transgenic lines (G1–G3). A, RNA-blot analysis. Total RNA (10 μg) was isolated and hybridized with a specific FsGASA4 probe. Bottom: Ethidium bromide-stained gel showing rRNAs. B, Southern-blot analysis. Genomic DNA was digested with HindIII, blotted onto a nylon membrane, and hybridized with a FsGASA4-specific probe.
