Figure 1.

Northern-blot analysis of AnnAt1 mRNA level after different treatments in Arabidopsis Col-0 plants. A, Detached, fully expanded leaves of 6-week-old plants were incubated on the surface of the following solutions (water [mock], 100 μm ABA, 150 mm NaCl, 1 mm salicylic acid [SA], 10 mm H₂O₂, and 100 mm Cd²⁺). To wound them, leaves were squeezed with grooved forceps five times per leaf. Total RNAs were isolated and hybridized with cDNA probes derived from the 3# untranslated region (764–1,116 nucleotides of the ORF) of AnnAt1 and actin 2 cDNA (1,062–1,283 nucleotides of the ORF). This experiment was performed twice and gave similar results. B, Detached, fully expanded leaves were subjected to drought (as described in “Materials and Methods”). Total RNAs were isolated and hybridized with AnnAt1 probe, as in A. As a loading control, 28S rRNA after staining with 0.2% (w/v) methylene blue is shown. The percentage of water loss at each time point is indicated at bottom. This experiment was performed twice and gave similar results.