Table I.

Physicochemical characterization of purified enterocin 62-6 against the sensitive Lactobacillus indicator strain 4-1 and the resistant isolate 62-5.

Inhibitory activity of enterocin 62-6 following exposure to	Growth inhibition of Lactobacillus indicator strain*
	4-1	62-5
No treatment, positive control	+	−
Heat, 100°C for 30 min	+	−
Trypsin (pH 7)	+	−
Pepsin (pH 3 and 7)	−	−
α-Chymotrypsin (pH 8)	−	−
Proteinase K (pH 7)	+	−
Lysozyme	+	−
Lipase	+	−
pH 4	+	−
pH 6	+	−
pH 8	+	−

Assays for inhibitory activity were carried out on MRS agar using the well diffusion technique.

^*As assessed by the measurement of the diameter (mm) of the zone of inhibited growth of the indicator strain, inclusive of the 5 mm diameter of the well, where ‘+’ indicates a diameter of the zone of growth inhibition of ≥7 mm and ‘−’ indicates a diameter of inhibited growth of <7 mm.