Table I.
Total cell yields in pulmonary lavage fluid from 7 piglets
| Pig ID | PBS wash (mL) | Fluid recovered (mL) | Cells/mLa |
|---|---|---|---|
| 1 | 1000 | 600 | 2 × 107 |
| 2 | 1000 | 750 | 8 × 107 |
| 3 | 1000 | 700 | 2 × 107 |
| 4 | 1000 | 500 | 1 × 107 |
| 5 | 1000 | 650 | 2 × 107 |
| 6 | 1000 | 750 | 3 × 107 |
| 7 | 1000 | 700 | 5 × 106 |
| Average | 1000 | 665 | 2.3 × 107 |
PBS — phosphate-buffered saline.
a
After washing, cells were resuspended in 100 mL of PBS. The total average number of cells collected was 2.3 × 107 × 100 mL, or 2.3 × 109. All batches of lavage cells collected supported the growth of Porcine reproductive and respiratory syndrome virus (PRRSV).