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Act1-dependent functions. (A) Activation of NF-κB. 293-TK/Zeo cells with and without Act1 were either untreated or treated with IL-1 for 15 min. Cell extracts were used for the gel-shift assays. (B) Activation of JNK. 293-TK/Zeo cells transfected with CMV-Act1 were untreated or treated with IL-1 (100 units/ml) or UV radiation (40 J/m) (2). Cell extracts were analyzed after Western transfer by using anti-phospho-JNK. Untransfected wild-type cells were used as controls.
