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Effect of a NIK dominant-negative mutant on the activation of NF-κB by Act1. Increasing amounts (50–500 ng) of NIK-DN were cotransfected with Act1 and E-selectin-Luc into 293-TK/Zeo cells. The effect of NIK-DN on Act1-induced E-selectin promoter activity was compared with its effect on IL-1-induced promoter activity.
