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. 2000 Aug 29;97(19):10489–10493. doi: 10.1073/pnas.160265197

Figure 5.

Figure 5

Interaction of Act1 with IKK. (A) IκB kinase assay. 293-TK/Zeo cells with or without Act1 transfection were untreated or treated with IL-1 for 15 min. Cell extracts were immunoprecipitated with anti-IKKγ, followed by a kinase assay using GST-IκB (amino acids 1–55) as substrate. (B) Interaction of Act1 with IKKγ. 293-TK/Zeo cells with or without expression of Act1-flag were untreated or treated with IL-1 for 3 min. Cell extracts were immunoprecipitated with anti-IKKγ and probed with an Ab directed against the flag tag.