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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Neuroscience. 2009 Apr 17;161(4):1082–1090. doi: 10.1016/j.neuroscience.2009.04.031

Figure 4.

Figure 4

Effect of section interval and number of sampling sites on neuron number estimation in SNpc. (A) Using an oversampling technique described by Slomianka and West (Slomianka and West, 2005) we determined the estimated deviation of dopaminergic neurons within the SNpc using different sampling intervals in a single C57BL/6J mouse. Counting every section through every 6th section gives numerical estimates within 10% (B) Using the same oversampling technique we estimated that an investigator could sample up to every third site and generate estimates within 10%.