Table 3.
Previous Studies of β-HPV DNA detection in NMSC tumor samples of immunocompetent individuals
| Author (year) | Sample size | Methods1 | β-HPV types detected | Main findings |
|---|---|---|---|---|
| Forslund et al. (2007) | 82 SCCs 126 BCCs |
Single and nested PCR of L1 region using CP65/CP70 and FAP primers2 | 8, 9, 12, 14, 15, 19, 20, 21, 23, 24, 37, 38, 80, 93, 96 | Association between β-species 2 and SCC |
| Pfister et al. (2003) | 20 SCCs | Nested PCR of L1 region using CP62/CP70a and CP65/CP69a primers | 5, 8, 12, 14, 15, 19, 21, 24, 25, 37, 38 | 80% of AK and 40% of SCC lesions were positive for βHPV DNA |
| Iftner et al. (2003) | 72 SCCs 18 BCCs |
PCR of E1 region using CP4/CP5 and PPF1/CP5 primers | 5, 8, 12, 17, 19, 22, 36 | Association between β-HPV DNA and NMSC (OR 6.4) |
| Boxman et al. (2000) | 14 BCCs | Nested PCR of the L1 region using CP62/CP70a and CP65/CP69a primers | 5, 8, 12, 14, 15, 17, 25,37, 38 | 43% of BCC tumors were β-HPV DNA-positive |
| Surentheran et al. (1998) | 6 SCCs | Degenerate and nested PCR of the L1 region using HPV2/B5, F14/B15, MY09/11, CP62/69 | 5, 8, 19, 20, 21, 23, 36 | All SCC samples were negative most likely due to low viral copy number |
AK, actinic keratoses; BCC, basal cell carcinoma; CI, confidence interval; HPV, human papillomavirus; NMSC, non-melanoma skin cancer; OR, odds ratio; SCC, squamous cell carcinoma
1
All PCR-based methods were followed by direct sequencing.
2
Pooled results from three laboratories using different PCR methods.