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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Cancer Res. 2009 Feb 3;69(4):1350–1357. doi: 10.1158/0008-5472.CAN-08-1328

Fig. 1. Autocrine loop of IGF-1R in PCA.

Fig. 1

Total cell lysates from AsPC-1 cells (A) treated with 1.0, 5.0 and 10.0 µg of anti-IGF-IRα 1H7 or mouse IgG (control); (B) treated with PPP (IGF-1R inhibitor) for different concentrations for 24h; (C) and (D) pretreated with protesome inhibitor (34) were resolved by SDS-PAGE and analyzed by immunoblotting with anti-IGF-1R 2C8 (IGF-IR). β-actin served as control protein.