Insulitis scores were rated as, Grade 0, no insulitis (white bars); grade 1, peri-insulitis (dotted bars); grade 2, insulitis in <50% of the islet (stripped bars); grade 3, insulitis in >50% of the islet (black bars) [39]. The percentage of islets in each scoring category is shown. All 10 DT, DNA-immunized animals developed Grade 2 and 3 insulitis. Only two of ten protein-immunized DT animals developed Grade 2 insulitis and one these mice had both grade 2 and 3 insulitis. All other groups had sporadic or no Grade 2 or 3 insulitis. * Indicates statistically significant differences (p ≤ 0.005) within the same grade, as compared to controls and all other groups.