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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Autoimmun. 2009 Mar 16;33(1):50–57. doi: 10.1016/j.jaut.2009.02.004

Figure 3. Humoral immunity in immunized mice.

Figure 3

GAD65 antibodies in DNA or protein immunized mice. Animals were named by a number and the first initial of their gender (f or m). A world standard control for measurement of GAD65 antibodies (GAD6 mMAb) was used as a positive control in all immunoprecipitation experiments. The results were transformed to reactivity units (RU) by defining the maximum amount of GAD65 radioactivity precipitated by GAD6 mMAb as 100%. The experiments were carried out in duplicate and repeated at least three times. The antibody levels of hGAD65 protein-immunized animals (right panel) were consistently higher than those of the hGAD65 DNA-immunized group (left panel). Not all animals developed detectable antibodies in either group (5 of 10 in DNA-immunized vs. 7 of 10 in protein-immunized groups).