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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Autoimmun. 2009 Mar 16;33(1):50–57. doi: 10.1016/j.jaut.2009.02.004

Figure 4. Cellular immunity in immunized mice.

Figure 4

T-cell proliferation assays were performed using peripheral blood mononuclear cells (PBMCs, left panel) and splenocytes (right panel). DNA-immunized animals’ results shown. Radioactivity from radiolabeled thymidine incorporation to proliferating T cells is expressed in CPM × 103 (Y axis, left margin). DT indicates double transgenics (DQ8-RIP7-hGAD65) while ST indicates single transgenics (GAD65 ST shown). None = no antigen added (negative control), Anti-CD3 was used to stimulate proliferation (positive control). Mean and standard deviation of triplicate results from 5 animals in each group (double or single transgenics) is shown. All double-transgenic animals tested had significant central (splenocytes) antigen specific proliferative responses to hGAD65 (top right) when compared to single-transgenic mice (bottom right).