Figure 6.

Distinct domains of CIKS required for activation of NF-κB and SAPK/JNK. CIKS mutants ΔN 301, ΔC 200, and ΔC 300 are truncations lacking the amino-terminal 301 aa, the carboxyl-terminal 200 aa, or the carboxyl-terminal 300 aa, respectively. The ability of these deletion mutants to interact with NEMO/IKKγ, IKKα, and IKKβ was assayed in coimmunoprecipitation experiments of transfected HeLa cells, as described for Fig. 3. The ability of these deletion mutants to activate NF-κB and SAPK/JNK was assayed with luciferase assays (as described for Fig. 4) and by immunecomplex kinase assay (as described in Fig. 5), respectively. +, positive assay; −, negative assay; *, a marginal effect in at least some assays.