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. Author manuscript; available in PMC: 2009 Jul 2.
Published in final edited form as: J Mol Biol. 2008 Jul 2;381(4):1025–1039. doi: 10.1016/j.jmb.2008.06.074

Fig. 6.

Fig. 6

ActRIIA also displaces the BMP-7 pd. (a) Sucrose gradient of ActRIIA together with the BMP-7 gfd. Immunoblotting with anti-BMP-7 gfd showed a main peak in fractions 8 and 9, indicating the formation of a receptor-gfd complex, (b) Interaction of BMP-7 complex with ActRIIA. Immunoblotting of sucrose gradient fractions revealed the appearance of four peaks: free complex (fractions 11–14); complex bound to the receptor molecule (fractions 10–12); free gfd bound to the receptor (fractions 7–9); and high-molecular-weight complexes, caused by dimerization of the Fc domains of the receptor molecules, possibly consisting of two BMPRII-Fc dimers and multiple BMP-7 gfds (fractions 3–5).