Skip to main content
. 2009 Jun 30;4(6):e6095. doi: 10.1371/journal.pone.0006095

Figure 1. TRPM8 channels are present in human sperm.

Figure 1

A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were used for TRPM8. Identity of the expected size (in white numbers) fragment was confirmed by DNA sequencing. B) Immunocytochemistry showing the presence of TRPM8 in heads and flagella of human sperm. Left panels show the confocal fluorescent images with pseudocolor and right panels show their corresponding phase contrast images. C) Western blot experiments showing TRPM8 reactivity (∼130 kDa) in sperm lysates (anti TRPM8 from Gene Tex). n≥3.