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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: J Mol Biol. 2009 Apr 10;389(2):306–314. doi: 10.1016/j.jmb.2009.04.013

Figure 3. Analytical ultracentrifugation of scUROD.

Figure 3

Samples of scUROD at 0.25 and 0.08 mg/mL were placed in two chambers of the centrifuge cell of a Beckman XL-A and spun at 12,000 rpm at 20° C to equilibrium. All data were fit globally to a monomer, resulting in a predicted molecular mass of 85,972 Da, consistent with the expected mass of the scUROD of 85,497 Da. (A) Points represent 268 averaged data values from the 5 scans (A280). The solid line is the best globally fit model. (B) Residuals for the scan shown in panel A.