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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: J Mol Biol. 2009 Apr 10;389(2):306–314. doi: 10.1016/j.jmb.2009.04.013

Figure 5. Product profile of competition assays using wild-type URO-D. (A).

Figure 5

The URO-D reaction was started with 5 μM uro'gen-I, at 5 min 45 μM uro'gen-III was added and 1 min later as aliquot was removed and the product profile was measured by HPLC (black trace). The reaction was allowed to continue and samples were removed for analysis at 5 min (blue trace), 10 min (green trace) 20 min (light blue trace), 30 min (pink trace) and 60 min (brown trace). The metabolites heptacarboxyl-porphyrin (7), hexacarboxyl-porphyrin (6), pentacarboxyl-porphyrin (5) and coproporphyrin (4) are labeled with the corresponding isomer designation (I or III) are labeled. (B) The total reaction products, in pmol, for isomer I (blue) and isomer III (red) at time 1, 5, 10, 20, 30 and 60 min are shown, error bars represent 3 independent samples. (C and D) Data from an identical experiment where 5 μM uro'gen-III was used initially, followed by the addition of 45 μM uro'gen-I.