Table 1. Activity of the scURO-D proteins.
| Uro'gen-I Activity | |||||
|---|---|---|---|---|---|
| Construct | # | activity | std err | % URO-D | % scURO-D |
| URO-D | 6 | 4364 | 188 | 100.0 | 133.9 |
| Y164G URO-D | 3 | 353 | 49 | 8.1 | 10.8 |
| F217Y URO-D | 4 | 114 | 18 | 2.6 | 3.5 |
| scURO-D | 6 | 3260 | 143 | 74.7 | 100.0 |
| scURO-D(FY) | 6 | 1802 | 40 | 41.3 | 55.3 |
| scURO-D(YF) | 6 | 2014 | 128 | 46.2 | 61.8 |
| scURO-D(YY) | 4 | 107 | 5 | 2.5 | 3.3 |
| scURO-D(GY) | 2 | 578 | 13.2 | 17.7 | |
| Uro'gen-III Activity | |||||
| Construct | # | activity | std err | % URO-D | % scURO-D |
| URO-D | 6 | 8028 | 913 | 100.0 | 117.7 |
| scURO-D | 6 | 6821 | 662 | 85.0 | 100.0 |
| scURO-D(FY) | 6 | 4087 | 320 | 50.9 | 59.9 |
| scURO-D(YF) | 6 | 4229 | 330 | 52.7 | 62.0 |
| scURO-D(YY) | 4 | 1163 | 62 | 14.5 | 17.1 |
Uro'gen-I, uroporphyrinogen-I; Uro'gen-III, uroporphyrinogen-III at 30 nM # number of independent assays performed in duplicate activity- average activity as nmol product/hr/mg protein std err- standard error