Skip to main content
. Author manuscript; available in PMC: 2009 Jul 2.
Published in final edited form as: Oncogene. 2007 Oct 8;27(14):1971–1980. doi: 10.1038/sj.onc.1210839

Figure 1. Inverse relationship between expression of MTA1 and BRCA1.

Figure 1

A, RT-PCR analysis showing decreased BRCA1 and PARP1 expression in MTA1-overexpressing MCF7 clones (first and second panels, right lane) when compared with control cells. Actin expression was used as an internal control for both RNA samples (third panel). B, Northern blot analysis showing a drastic decrease in BRCA1 mRNA levels in MCF7/MTA1 cells when compared with MCF7/pcDNA (control) cells (first panel, second lane), thus implicating MTA1 over expression in the repression of BRCA1 gene transcription. C, Significant decreases in the protein levels of BRCA1 (left) and PARP1 (right) in the presence of MTA1 over expression (first panel, second lane). Actin or Vinculin expression was used as an internal control. D, Increase in BRCA1 levels as a result of MTA1 knockdown with small interfering RNA (siRNA) (first panel, right lane). A decreased level of MTA1 expression (second panel, right lane) shows effective knockdown of MTA1 by MTA1 siRNA. Actin expression was used as an internal control (third panel). Expression levels were quantified using the ImageQuant version 5.1 program.