Fig. 3.

Mean ± SE of labelling density (gold grains/µm²) on different subcellular compartments of hepatocytes from euthermic, hibernating and arousing hazel dormice with antibodies directed against FAS and ACSL1. The density values of anti-FAS labelling in mitochondria from euthermic and hibernating dormice were not significantly different from background (P = 0.702 and P = 0.779, respectively). Similarly, anti-FAS labelling in cell nuclei showed values similar to background in all seasonal phases (P = 0.861 in euthermia; P = 0.385 in hibernation; P = 0.231 upon arousal). Tukey's HSD test for multiple comparisons applied to the ‘cellular compartment’ (cytoplasm, mitochondria, nucleus) factor revealed significant differences for both FAS (euthermia: P < 0.001 for cytoplasm vs. mitochondria and cytoplasm vs. nucleus; hibernation: P = 0.045 for cytoplasm vs. mitochondria and P = 0.006 for cytoplasm vs. nucleus; arousal: P = 0.002 for cytoplasm vs. mitochondria, P = 0.016 for cytoplasm vs. nucleus and P < 0.001 for mitochondria vs. nucleus) and ACSL1 (euthermia: P = 0.041 for cytoplasm vs. nucleus and P = 0.036 for mitochondria vs. nucleus; hibernation: P < 0.001 for all comparisons). The values that do not differ significantly from each other are indicated by the same letter (a,b). Tukey's HSD test applied to the ‘seasonal phase’ (euthermia, hibernation, arousal) factor revealed significant differences for both FAS (cytoplasm: P < 0.001 for euthermia vs. hibernation and arousal, and P = 0.005 for hibernation vs. arousal; mitochondria:P < 0.001 for arousal vs. euthermia and hibernation) and ACSL1 (cytoplasm: P < 0.001 for euthermia vs. hibernation, P = 0.002 for euthermia vs. arousal and P = 0.008 for hibernation vs. arousal; mitochondria and nucleus: P < 0.001 for euthermia vs. hibernation and arousal). The values that do not differ significantly from each other are indicated by the same letter (c,d).