Table 1.
Initial enzyme activity after loading enzymes and processing into silk films
| Enzyme | Treatment | Total amount loaded (wt%) | Activity loadeda (unit/mg film) | Activity measuredb(unit/mg film) | Activity retentionb (%) |
|---|---|---|---|---|---|
| GOx | No MeOH |
1 | 0.21 | 0.16±0.03 | 75.2±14.7 |
| 0.1 | 0.021 | 0.028±0.002 | 131±14 | ||
| MeOH | 1 | 0.21 | 0.0027±0.001 | 1.26±0.64 | |
| 0.1 | 0.021 | 0.0029±0.006 | 13.8±2.6 | ||
| Lipase | No MeOH |
1 | 7 | 3.01±0.84 | 43.0±11.5 |
| 0.1 | 0.7 | 0.58±0.24 | 82.6±35.0 | ||
| MeOH | 1 | 7 | 0.70±0.38 | 10.3±5.5 | |
| 0.1 | 0.7 | 0.38±0.07 | 53.9±7.6 | ||
| HRP | No MeOH |
1 | 10.8 | 25.6±2.6 | 237±52 |
| 0.1 | 1.08 | 13.2±0.11 | 122±9 | ||
| MeOHc | 0.1 | 1.08 | 0.33±0.11 | 31.9±8.4 |
a
Enzyme activity values (units) were taken from manufacturer’s data sheet and it was assumed that no activity was lost prior to use.
b
Values are average ± standard derivation of N=5 samples.
c
The activity could not be measured in the case of methanol treated film containing 1 wt% HRP because the enzyme concentration was too high to measure, even at the smallest film size and largest substrate volume.