Skip to main content
. 2000 Sep 5;97(19):10503–10508. doi: 10.1073/pnas.180169797

Figure 3.

Figure 3

(A) Complementation studies with purified CSB protein. Before the transcription reaction, no (lanes 1, 4) or increasing amounts of purified CSB protein [3 ng (lanes 3, 6) and 30 ng (lanes 2, 5)] were added to a nuclear extract prepared from mock-treated or UV-irradiated (5 J/m2) CSIAN cells 6 h after UV exposure. (B) Transcription activity in mixed extracts. (i) Extracts from UV-irradiated CS1AN and CS3BE cells prepared 6 h after UV exposure were mixed and assayed for transcription activity (lanes 1 and 6, nonirradiated CS1AN and CS3BE, respectively; lanes 2 and 5, UV irradiated (5 J/m2 CS1AN and CS3BE, respectively); and lanes 3 and 4, mix of equal amounts of UV-irradiated CS1AN and CS3BE). (ii) Different quantities of MRC5 and CS1AN extracts [prepared 6 h after UV treatment (5 J/m2)] were mixed and assayed for transcription activity (lanes 7 and 12, equal amounts of UV-irradiated MRC5 and CS1AN, respectively; lanes 8–11, mix of various amounts of MRC5, CS1AN, and buffer).