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. Author manuscript; available in PMC: 2009 Jul 2.
Published in final edited form as: J Mol Biol. 2008 Feb 29;379(3):492–504. doi: 10.1016/j.jmb.2008.02.049

Figure 1.

Figure 1

Interaction and genomic localization of Dnmt3b and Tdg in EC cells. (a) Endogenous Dnmt3b was immunoprecipitated from F9 EC cell nuclear extracts cells with increasing concentrations (0.5, 1.0, 2.0 μg) of α-Dnmt3b antibody. An immunoblot of the pull down product with α-Tdg antibody confirmed that Tdg coprecipitated with Dnmt3b. (b) Genomic localization of Dnmt3b and Tdg by chromatin immunoprecipitation (ChIP). Dnmt3b and Tdg were immunoprecipitated from crosslinked chromatin in P19 EC cells. PCR was performed using primers designed to amplify the centromeric minor satellite repeats. (b′) Dnmt3b and Tdg were immunoprecipitated from crosslinked chromatin. The complexes were purified by several wash steps and subjected to a second ChIP (i.e. ReChIP) with the antibody indicated above. PCR was performed using primers designed to amplify the pericentromeric major satellite repeats or the intracisternal A particle long terminal repeats (IAP LTR). Control PCR reactions utilized total input DNA, DNA isolated using species-matched normal IgG antibody and DNA isolated in the absence of immunoprecipitating antibody.